A Red-Dot-Blot Protein Assay Technique in the Low Nanogram Range
نویسندگان
چکیده
منابع مشابه
A red-dot-blot protein assay technique in the low nanogram range.
A simple, sensitive, rapid (3 min), and highly reproducible solid-phase assay for the detection of proteins in the low nanogram range (4 ng) is described. The assay is based on differential Ponceau S staining of the protein spots on nitrocellulose and quantification of the protein-dye complexes on lubricated membranes using a densitometer. The dye solution used for protein staining contained 0....
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1999
ISSN: 0003-2697
DOI: 10.1006/abio.1999.4057